Journal: Toxics

Article Title: In Vitro Evaluation of the Effects of Cadmium on Endocytic Uptakes of Proteins into Cultured Proximal Tubule Epithelial Cells

doi: 10.3390/toxics8020024

Figure Lengend Snippet: Fluorescence imaging of endocytosed proteins in mouse proximal tubule epithelial cell (PTEC)-derived S1 cells. ( A ) S1 cells were incubated with 50 µg/mL fluorescein isothiocyanate (FITC)-albumin, Alexa-transferrin, FITC-β 2 -MG, or FITC-MT (green) for 30 min and then fixed with paraformaldehyde for immunofluorescence labeling with anti-EEA1 (red). Yellow staining demonstrates the colocalization of fluorescent-labeled proteins and early endosomes. ( B ) S1 cells were incubated with Alexa-transferrin for 1, 5, 10, 15, and 30 min. The localization of Alexa-transferrin (green) and early endosomes stained with anti-EEA1 (red) was visualized by confocal microscopy. Bars, 5 µm.

Article Snippet: Rabbit anti-transferrin receptor polyclonal antibody was purchased from Abnova (Taipei, Taiwan).

Techniques: Fluorescence, Imaging, Derivative Assay, Incubation, Immunofluorescence, Labeling, Staining, Confocal Microscopy

Journal: Toxics

Article Title: In Vitro Evaluation of the Effects of Cadmium on Endocytic Uptakes of Proteins into Cultured Proximal Tubule Epithelial Cells

doi: 10.3390/toxics8020024

Figure Lengend Snippet: Effects of Cd on the endocytosis efficiencies of albumin and transferrin into S1 cells. S1 cells were exposed to CdCl 2 for 1, 3, and 6 days and then incubated with FITC-albumin ( A ) or Alexa-transferrin ( B ) for 30 min. The endocytosis efficiencies were determined by flow cytometry and expressed as percentages of the control cells (no exposure to Cd). Data are presented as means ± SD ( n = 3–4).

Article Snippet: Rabbit anti-transferrin receptor polyclonal antibody was purchased from Abnova (Taipei, Taiwan).

Techniques: Incubation, Flow Cytometry, Control